The Cambridge group employs a broad multi-disciplinary approach that combines methods and ideas ranging from physical-organic chemistry to biophysics, molecular biology and directed evolution. High- and low-throughput approaches are combined with classical kinetic and thermodynamic analysis. For metagenomic screening the group has developed the ability to carry out screening of up to 10 clones in microfluidic devices used to make droplets that allow assays in picolitre compartments, representing a 10-fold reduction in assay volume and a corresponding reduction in cost. This emerging technology is ideally suited to high-throughput screening to make protein reagents for green production lines and synthetic biology.
In BlueRemediomics, UCAM is contributing to work packages 2 and 3.